Increased anatomical detail by in vitro MR microscopy with a modified Golgi impregnation method

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Increased anatomical detail by in vitro MR microscopy with a modified Golgi impregnation method.

Golgi impregnation is unique in its ability to display the dendritic trees and axons of large numbers of individual neurons by histology. Here we apply magnetic resonance microscopy to visualize the neuroanatomy of animal models by combining histologic fixation chemistry with paramagnetic contrast agents. Although there is some differential uptake of the standard small-molecular-weight contrast...

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A Modified Golgi Impregnation method for In Vitro MR Microscopy

X. Zhang, R. E. Jacobs California Institute of Technology, Pasadena, CA, United States In the late 19 century Camillo Golgi revolutionize the budding discipline of neuroscience with his Black Reaction [1, 2], now known as the Golgi stain. This reaction stains a small eclectic, likely random, portion of neurons and processes in the tissue of interest. The Golgi stain consists of pretreatment wit...

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ژورنال

عنوان ژورنال: Magnetic Resonance in Medicine

سال: 2010

ISSN: 0740-3194

DOI: 10.1002/mrm.22322